pEASY®-Basic Seamless Cloning and Assembly Kit
基础版同源重组无缝克隆试剂盒
| 目录号 | 规格 | 单价 |
|---|---|---|
| CU201-02 | 20 rxns | 680 |
| CU201-03 | 60 rxns | 1830 |
产品详情介绍
本产品利用特殊的重组酶和同源重组的原理，可以将任意方法线性化后的载体和与其两端具有15-25 bp重叠区域
的PCR片段定向重组，可以实现1-5个片段的高效无缝拼接。
• 快速：仅需要5~15分钟反应时间。
• 简单：不受片段酶切位点的影响，无需对片段酶切。
• 高效：阳性率达95% 以上。
• 无缝：不引入额外的序列。
1 Zou Z P, Du Y, Fang T T, et al. Biomarker-responsive engineered probiotic diagnoses, records, and ameliorates inflammatory bowel disease in mice[J]. Cell Host & Microbe, 2023.(IF 31.31)
2 Wan W, Dong H, Lai D H, et al. The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments[J]. Nature communications, 2023.(IF 17.69)
3 You L, Omollo E O, Yu C, et al. Structural basis for intrinsic transcription termination[J]. Nature, 2023.(IF 69.50)
4 Zhang H W, Huang K, Gu Z X, et al. A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex[J]. Nature Communications, 2023.(IF 17.69)
5 Huang J, Yang L, Yang L, et al. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae[J]. Nature, 2023(IF 64.80)
6 Tang J, Zhang G, Li F, et al. Two-Dimensional Protein Nanoarray as a Carrier of Sensing Elements for Gold-Based Immunosensing Systems[J]. Analytical Chemistry, 2022.(IF 8.00)
7 Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 63.71)
8 Chen Q, Hu T, Li X, et al. Phosphorylation of SWEET sucrose transporters regulates plant root: shoot ratio under drought[J]. Nature plants, 2022.(IF 15.79)
9 Liu R, Yang J, Yao J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins[J]. Nature biotechnology, 2022.(IF 54.90)
10 Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 63.71)
11 Zeng Y H, Cai Z H, Zhu J M, et al. Two hierarchical LuxR-LuxI type quorum sensing systems in Novosphingobium activate microcystin degradation through transcriptional regulation of the mlr pathway[J]. Water Research, 2020.(IF 9.13)
