TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix
TransScript一步法去除gDNA及第一链cDNA合成试剂盒
| 目录号 | 规格 | 单价 |
|---|---|---|
| AT311-02 | 50 rxns×20 µl体系 | 1160 |
| AT311-03 | 100 rxns×20 µl体系 | 2080 |
| AT311-04 | 500 rxns×20 µl体系 | 9350 |
产品详情介绍
本产品以RNA为模板，在同一反应体系中，合成第一链cDNA的同时去除RNA模板中残留的基因组DNA。反应结束后，只需在85℃加热5秒钟，即可同时失活TranScript® RT/RI与gDNA Remover。
• 在同一反应体系中，同时完成反转录与基因组DNA的去除，操作简便，降低污染机率。
• 产物用于qPCR：反转录15分钟；产物用于PCR：反转录30分钟。
• 反应结束后，同时热失活RT/RI与gDNA Remover。与传统的用DNase I预处理RNA的方法相比，避免了处理后热失活DNase I对RNA的损伤。
• 操作简单。
•合成片段≤12 kb。
使用前请将各组分点甩离心
产品稳定性
使用不同批次产品分别以人总RNA为模板，进行RT-PCR检测，1.0%琼脂糖凝胶电泳分析反转录效果。
使用不同批次产品分别以人100 ng总RNA、人100 ng总RNA+200 ng gDNA、200 ng gDNA为模板，进行RT-PCR检测，1.0%琼脂糖凝胶电泳分析模板DNA去除效果；qRT-PCR检测18S RNA表达量。
与竞品的比较
使用TransGen、Company TA和Company TH产品，分别以人100 ng总RNA、人100 ng总RNA+200 ng gDNA、200 ng gDNA为模板，进行RT-PCR检测， 1.0%琼脂糖凝胶电泳分析模板DNA去除效果；qRT-PCR检测18S RNA表达量。
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